Brucella abortus RB51 and Hot Saline Extract from Brucella ovis as Antigens in a Complement Fixation Test Used To Detect Sheep Vaccinated with Brucella abortus RB51
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Brucella abortus RB51 and hot saline extract from Brucella ovis as antigens in a complement fixation test used To detect sheep vaccinated with Brucella abortus RB51.
The efficacy of Brucella abortus RB51 and hot saline extract (HSE) from Brucella ovis as antigens in complement fixation (CF) tests was comparatively evaluated in detecting immune responses of sheep vaccinated with B. abortus strain RB51. For this study, four 5-month-old sheep were vaccinated subcutaneously with 5 x 10(9) CFU of RB51, and two sheep received saline. Serum samples collected at di...
متن کاملComplement fixation test to assess humoral immunity in cattle and sheep vaccinated with Brucella abortus RB51.
The live attenuated Brucella abortus strain RB51 is a rifampin-resistant, lipopolysaccharide (LPS) O-chain-deficient mutant of virulent B. abortus 2308. The reduced O-chain content in RB51 prevents this bacterium from inducing antibodies detectable by the conventional serologic tests for bovine brucellosis diagnosis that mainly identify antibodies to LPS. The absence of available serologic test...
متن کاملAntigenic relationship of Brucella ovis to Brucella abortus and Brucella melitensis using the complement fixation test.
WORTHINGTON, R. W. & MULDERS, MARIAS. G. Antigenic relationship of Brucellaovis to Brucella abortus and Brucella melitensis using the complement fixation test. Onderstepoort J. vet. Res., 36 (2) , 191-1 98, 1969. The CF test was used to investigate the serological relationship of Br. avis to Br. abortus and Br. melitensis. A definite antigenic relationship between Br. avis and Br. abortus could...
متن کاملImmune Response of Calves Vaccinated with Brucella abortus S19 or RB51 and Revaccinated with RB51
Brucella abortus S19 and RB51 strains have been successfully used to control bovine brucellosis worldwide; however, currently, most of our understanding of the protective immune response induced by vaccination comes from studies in mice. The aim of this study was to characterize and compare the immune responses induced in cattle prime-immunized with B. abortus S19 or RB51 and revaccinated with ...
متن کاملSafety of Brucella abortus strain RB51 in black bears.
In two studies conducted from October 1999 to March 2000 and December 2000 to April 2001, adult black bears (Ursus americanus) were orally inoculated with 1.4-3.1 x 10(10) colony-forming units (CFU) of Brucella abortus strain RB51 (SRB51, n=12) or 2 ml of 0.15 M NaCl solution (saline, n=11). We did not detect a difference (P>0.05) in antibody titers to SRB51 in serum obtained before vaccination...
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ژورنال
عنوان ژورنال: Clinical Diagnostic Laboratory Immunology
سال: 2001
ISSN: 1071-412X,1098-6588
DOI: 10.1128/cdli.8.1.119-122.2001